Through our experiments, and further research, our original hypothesis was proven false. The growth rate of S. cerevisiae is not severely stunted by a single knockout of the gene SDH4. However, with further research, Kubo et al. conducted research showing that a double knockout of SDH4 (knocking out SDH1-4 and the sub gene SDH4b) deactivates the original gene as well as the sub gene that becomes activated in the absence of SDH4. This double knockout showed severely stunted growth in Kubo’s et al. research.
In regards to the novel analytical platforms, the petri dish-pipette tip model showed the most success and signs of growth. Thicker barriers were also more helpful in creating barriers and wax paper was incredibly cost efficient, as well as easily accessible; PDMS molds were shown to be cost efficient as well, as we used the same mold for both trials. The molds are easily washed out once an experiment is completed.
Our group would like to thank Dr. Van Dyken and Dr. Knecht for the instruction they have provided for us during this semester and guiding us. We would also like to thank the Howard Hughes Medical Institute for providing the program and opportunity of conduct this research. Last but not least, we would also like to thank Lily Acheapomaa-Piasare, Yi Wen and Reid Collis for assisting and guiding us in all our research endeavors.